SDS Page:Article Title: Degradation of Nonribosomal Peptide Synthetase Megasynthetases SrfAA and SrfAB by Acyldepsipeptide‐Activated ClpP in Bacillus Subtilis
Article Snippet: Full images of SDS-PAGE gels from Figure 3. (A) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (B) Labeling of SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2. (C) Labeling of SrfAC and LeuRS in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (D) Labeling of SrfAA and SrfAB in cell lysates from the untreated control using the ABPP probe 3. (E) Labeling of SrfAB in cell lysates from the untreated control using the ABPP probe 2. (F) Inhibition studies of SrfAA and SrfAB labeling in cell lysates from ADEP1-treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3 and Leu-AMS. (G) Inhibition studies of SrfAB labeling in cell lysates from ADEP1treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2 and AspAMS. (H) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 3. (I) Labeling of SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 2.
Labeling:Article Title: Degradation of Nonribosomal Peptide Synthetase Megasynthetases SrfAA and SrfAB by Acyldepsipeptide‐Activated ClpP in Bacillus Subtilis
Article Snippet: Full images of SDS-PAGE gels from Figure 3. (A) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (B) Labeling of SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2. (C) Labeling of SrfAC and LeuRS in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (D) Labeling of SrfAA and SrfAB in cell lysates from the untreated control using the ABPP probe 3. (E) Labeling of SrfAB in cell lysates from the untreated control using the ABPP probe 2. (F) Inhibition studies of SrfAA and SrfAB labeling in cell lysates from ADEP1-treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3 and Leu-AMS. (G) Inhibition studies of SrfAB labeling in cell lysates from ADEP1treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2 and AspAMS. (H) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 3. (I) Labeling of SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 2.
Control:Article Title: Degradation of Nonribosomal Peptide Synthetase Megasynthetases SrfAA and SrfAB by Acyldepsipeptide‐Activated ClpP in Bacillus Subtilis
Article Snippet: Full images of SDS-PAGE gels from Figure 3. (A) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (B) Labeling of SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2. (C) Labeling of SrfAC and LeuRS in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (D) Labeling of SrfAA and SrfAB in cell lysates from the untreated control using the ABPP probe 3. (E) Labeling of SrfAB in cell lysates from the untreated control using the ABPP probe 2. (F) Inhibition studies of SrfAA and SrfAB labeling in cell lysates from ADEP1-treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3 and Leu-AMS. (G) Inhibition studies of SrfAB labeling in cell lysates from ADEP1treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2 and AspAMS. (H) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 3. (I) Labeling of SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 2.
Inhibition:Article Title: Degradation of Nonribosomal Peptide Synthetase Megasynthetases SrfAA and SrfAB by Acyldepsipeptide‐Activated ClpP in Bacillus Subtilis
Article Snippet: Full images of SDS-PAGE gels from Figure 3. (A) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (B) Labeling of SrfAB in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2. (C) Labeling of SrfAC and LeuRS in cell lysates from ADEP1-treated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3. (D) Labeling of SrfAA and SrfAB in cell lysates from the untreated control using the ABPP probe 3. (E) Labeling of SrfAB in cell lysates from the untreated control using the ABPP probe 2. (F) Inhibition studies of SrfAA and SrfAB labeling in cell lysates from ADEP1-treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 3 and Leu-AMS. (G) Inhibition studies of SrfAB labeling in cell lysates from ADEP1treated and untreated wt B. subtilis strain 168 (ATCC 23857) cells using the ABPP probe 2 and AspAMS. (H) Labeling of SrfAA and SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 3. (I) Labeling of SrfAB in cell lysates from ADEP1-treated ΔclpP cells using the ABPP probe 2.
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